Please see our recommendations on RNA isolation.
Target Labelling, Purification and Array Hybridisation
Target labelling, purification and hybridisation protocol (Last updated October 2005 - changes from last version are highlighted in blue)
Note: For pre-hybridisation of the slides, hybridisation of the labelled targets on the probes and post-hybridisation washes we recommend the use of Pronto! Universal Microarray Hybridisation kit (Corning, Cat.# 40026) as described in the above protocol.
Lucidea Universal ScoreCard
PCR-array layouts SCp36 (and greater) and Oligo-array layouts include calibration, ratio, utility and negative control spots from the Lucidea Universal ScoreCard kit (GE Healthcare, formerly Amersham Biosciences) for optional use.
Some external sources of protocols: