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Professor Johnjoe McFadden

Professor of Molecular Genetics

Qualifications: BSc (Biochemistry), PhD (Biochemistry)

Email:
Phone: Work: 01483 68 6494
Room no: 07 AX 01

Further information

Biography

2002 – present, . Professor of Molecular Genetics, University of Surrey
1994 – 2001 Reader, School of Biological Sciences, University of Surrey.
1988-94 Lecturer, School of Biological Sciences, University of Surrey.
1984-88 Research Fellow. Department of Surgery, St. George's Hospital Medical School, London.
1982-84 Research Fellow, Department of Biochemistry, St. Mary's Hospital Medical School, London.

1982: PhD (Biochemistry) Imperial College, University of London

1978 BSc (Biochemistry) Bedford College, University of London

Research Interests

Systems Biology

Mycobacterial genetics

Pathogenicity of tuberculosis

Neisserial genetics

Pathogenicity of meningococcal meningitis

Bionanotechnology

Research Collaborations

CARBIO network: Marie Curie Research Training Network - Multifunctional Carbon Nanotubes for Biomedical Applications

Graham Stewart

Andrzej Kierzek

Publications

Highlights

  • Beste DJ, Bonde B, Hawkins N, Ward JL, Beale MH, Noack S, Nöh K, Kruger NJ, Ratcliffe RG, McFadden J. (2011) '¹³C metabolic flux analysis identifies an unusual route for pyruvate dissimilation in mycobacteria which requires isocitrate lyase and carbon dioxide fixation.'. PLoS PLoS Pathog, United States: 7 (7)
    doi: 10.1371/journal.ppat.1002091
    Full text is available at: http://epubs.surrey.ac.uk/184899/

Journal articles

  • Heister E, Neves V, Lamprecht C, Silva SRP, Coley HM, McFadden J. (2012) 'Drug loading, dispersion stability, and therapeutic efficacy in targeted drug delivery with carbon nanotubes'. Carbon,
    Full text is available at: http://epubs.surrey.ac.uk/7905/
  • Sanz V, Tilmacîu C, Soula B, Flahaut E, Coley HM, Silva SRP, McFadden J. (2011) 'Chloroquine-enhanced gene delivery mediated by carbon nanotubes'. Elsevier Carbon, 49 (15), pp. 5348-5358.
    doi: 10.1016/j.carbon.2011.08.001
    Full text is available at: http://epubs.surrey.ac.uk/7815/
  • Beste DJ, Bonde B, Hawkins N, Ward JL, Beale MH, Noack S, Nöh K, Kruger NJ, Ratcliffe RG, McFadden J. (2011) '¹³C metabolic flux analysis identifies an unusual route for pyruvate dissimilation in mycobacteria which requires isocitrate lyase and carbon dioxide fixation.'. PLoS PLoS Pathog, United States: 7 (7)
    doi: 10.1371/journal.ppat.1002091
    Full text is available at: http://epubs.surrey.ac.uk/184899/
  • Bonde BK, Beste DJV, Laing E, Kierzek AM, McFadden J. (2011) 'Differential Producibility Analysis (DPA) of Transcriptomic Data with Metabolic Networks: Deconstructing the Metabolic Response of M. tuberculosis'. PUBLIC LIBRARY SCIENCE PLOS COMPUT BIOL, 7 (6) Article number e1002060
    doi: 10.1371/journal.pcbi.1002060
    Full text is available at: http://epubs.surrey.ac.uk/184902/
  • Sanz V, Borowiak E, Lukanov P, Galibert AM, Flahaut E, Coley HM, Silva SRP, McFadden J. (2011) 'Optimising DNA binding to carbon nanotubes by non-covalent methods'. PERGAMON-ELSEVIER SCIENCE LTD CARBON, 49 (5), pp. 1775-1781.
    doi: 10.1016/j.carbon.2010.12.064
    Full text is available at: http://epubs.surrey.ac.uk/7818/
  • Sroka J, Bieniasz-Krzywiec L, Gwóźdź S, Leniowski D, Lącki J, Markowski M, Avignone-Rossa C, Bushell ME, McFadden J, Kierzek AM. (2011) 'Acorn: a grid computing system for constraint based modeling and visualization of the genome scale metabolic reaction networks via a web interface.'. BMC Bioinformatics, England: 12
    doi: 10.1186/1471-2105-12-196
    Full text is available at: http://epubs.surrey.ac.uk/185976/
  • Shi LB, Sohaskey CD, Pfeiffer C, Datta P, Parks M, McFadden J, North RJ, Gennaro ML. (2010) 'Carbon flux rerouting during Mycobacterium tuberculosis growth arrest'. WILEY-BLACKWELL PUBLISHING, INC MOL MICROBIOL, 78 (5), pp. 1199-1215.
    doi: 10.1111/j.1365-2958.2010.07399.x
  • Kadir TAA, Mannan AA, Kierzek AM, McFadden J, Shimizu K. (2010) 'Modeling and simulation of the main metabolism in Escherichia coli and its several single-gene knockout mutants with experimental verification'. BIOMED CENTRAL LTD MICROB CELL FACT, 9 Article number 88
    doi: 10.1186/1475-2859-9-88
    Full text is available at: http://epubs.surrey.ac.uk/239125/
  • Beste DJV, McFadden J. (2010) 'Systems biology of the metabolism of Mycobacterium tuberculosis'. PORTLAND PRESS LTD Biochemical Society Transactions, 38 (5), pp. 1286-1289.
    doi: 10.1042/BST0381286
    Full text is available at: http://epubs.surrey.ac.uk/184901/
  • McFadden J. (2010) 'Physics and the mind Comment on 'Natural world physical-, brain operational-, and mind phenomenal-space-time' by Fingelkurts et al.'. ELSEVIER SCIENCE BV PHYS LIFE REV, 7 (2), pp. 250-251.
    doi: 10.1016/j.plrev.2010.04.003
    Full text is available at: http://epubs.surrey.ac.uk/7370/
  • Heister E, Lamprecht C, Neves V, Tilmaciu C, Datas L, Flahaut E, Soula B, Hinterdorfer P, Coley HM, Silva SRP, McFadden J. (2010) 'Higher Dispersion Efficacy of Functionalized Carbon Nanotubes in Chemical and Biological Environments'. AMER CHEMICAL SOC ACS NANO, 4 (5), pp. 2615-2626.
    doi: 10.1021/nn100069k
    Full text is available at: http://epubs.surrey.ac.uk/7323/
  • Neves V, Heister E, Costa S, Tilmaciu C, Borowiak-Palen E, Giusca CE, Flahaut E, Soula B, Coley HM, McFadden J, Silva SRP. (2010) 'Uptake and Release of Double-Walled Carbon Nanotubes by Mammalian Cells'. WILEY-V C H VERLAG GMBH ADV FUNCT MATER, 20 (19), pp. 3272-3279.
    doi: 10.1002/adfm.201000994
  • Beste DJV, McFadden J. (2010) 'System-level strategies for studying the metabolism of Mycobacterium tuberculosis'. ROYAL SOC CHEMISTRY MOL BIOSYST, 6 (12), pp. 2363-2372.
    doi: 10.1039/c003757p
    Full text is available at: http://epubs.surrey.ac.uk/184934/
  • Borsuk S, Newcombe J, Mendum TA, Dellagostin OA, McFadden J. (2009) 'Identification of proteins from tuberculin purified protein derivative (PPD) by LC-MS/MS'. Elsevier TUBERCULOSIS, 89 (6), pp. 423-430.
    doi: 10.1016/j.tube.2009.07.003
    Full text is available at: http://epubs.surrey.ac.uk/7319/
  • Lamprecht C, Liashkovich I, Neves V, Danzberger J, Heister E, Rangl M, Coley HM, McFadden J, Flahaut E, Gruber HJ, Hinterdorfer P, Kienberger F, Ebner A. (2009) 'AFM imaging of functionalized carbon nanotubes on biological membranes'. IOP PUBLISHING LTD NANOTECHNOLOGY, 20 (43) Article number 434001
    doi: 10.1088/0957-4484/20/43/434001
  • Heister E, Neves V, Tilmaciu C, Lipert K, Beltran VS, Coley HM, Silva SRP, McFadden J. (2009) 'Triple functionalisation of single-walled carbon nanotubes with doxorubicin, a monoclonal antibody, and a fluorescent marker for targeted cancer therapy'. PERGAMON-ELSEVIER SCIENCE LTD CARBON, 47 (9), pp. 2152-2160.
    doi: 10.1016/j.carbon.2009.03.057
    Full text is available at: http://epubs.surrey.ac.uk/7324/
  • Beste DJV, Espasa M, Bonde B, Kierzek AM, Stewart GR, McFadden J. (2009) 'The Genetic Requirements for Fast and Slow Growth in Mycobacteria'. PUBLIC LIBRARY SCIENCE PLOS ONE, 4 (4) Article number e5349
    doi: 10.1371/journal.pone.0005349
    Full text is available at: http://epubs.surrey.ac.uk/184900/
  • Mendum TA, Newcombe J, McNeilly CL, McFadden J. (2009) 'Towards the Immunoproteome of Neisseria meningitidis'. PUBLIC LIBRARY SCIENCE PLOS ONE, 4 (6) Article number e5940
    doi: 10.1371/journal.pone.0005940
    Full text is available at: http://epubs.surrey.ac.uk/2561/
  • Senaratne RH, Sidders B, Sequeira P, Saunders G, Dunphy K, Marjanovic C, Reader JR, Lima P, Chan S, Kendall S, McFadden J, Riley LW. (2008) 'Mycobacterium tuberculosis strains disrupted in mce3 and mce4 operons are attenuated in mice'. SOC GENERAL MICROBIOLOGY J MED MICROBIOL, 57 (2), pp. 164-170.
    doi: 10.1099/jmm.0.47454-0
  • Casey R, Newcombe J, McFadden JJ, Bodman-Smith KB. (2008) 'The acute-phase reactant C-reactive protein binds to phosphorylcholine-expressing Neisseria meningitidis and increases uptake by human phagocytes'. AMER SOC MICROBIOLOGY INFECT IMMUN, 76 (3), pp. 1298-1304.
    doi: 10.1128/IAI.00741-07
    Full text is available at: http://epubs.surrey.ac.uk/351097/
  • Borsuk S, Mendum TA, Fagundes MQ, Michelon M, Cunha CW, McFadden J, Dellagostin OA. (2007) 'Auxotrophic complementation as a selectable marker for stable expression of foreign antigens in Mycobacterium bovis BCG'. CHURCHILL LIVINGSTONE TUBERCULOSIS, 87 (6), pp. 474-480.
    doi: 10.1016/j.tube.2007.07.006
  • Beste DJV, Laing E, Bonde B, Avignone-Rossa C, Bushell ME, McFadden JJ. (2007) 'Transcriptomic analysis identifies growth rate modulation as a component of the adaptation of mycobacteria to survival inside the macrophage'. AMER SOC MICROBIOLOGY J BACTERIOL, 189 (11), pp. 3969-3976.
    doi: 10.1128/JB.01787-06
    Full text is available at: http://epubs.surrey.ac.uk/184935/
  • Santangelo MP, McIntosh D, Bigi F, Armoa GRG, Campos ASD, Ruybal P, Dellagostin OA, McFadden J, Mendum T, Gicquel B, Winter N, Farber M, Cataldi A. (2007) 'Mycobacterium bovis BCG as a delivery system for the RAP-1 antigen from Babesia bovis'. ELSEVIER SCI LTD VACCINE, 25 (6), pp. 1104-1113.
    doi: 10.1016/j.vaccine.2006.09.069
  • Beste DJV, Hooper T, Stewart G, Bonde B, Avignone-Rossa C, Bushell M, Wheeler P, Klamt S, Kierzek AM, McFadden J. (2007) 'GSMN-TB: a web-based genome scale network model of Mycobacterium tuberculosis metabolism'. BIOMED CENTRAL LTD GENOME BIOL, 8 (5) Article number r89
    doi: 10.1186/gb-2007-8-5-r89
    Full text is available at: http://epubs.surrey.ac.uk/184904/
  • Michelon A, Conceicao FR, Binsfeld PC, da Cunha CW, Moreira AN, Argondizzo AP, McIntosh D, Armoa GRG, Campos AS, Farber M, McFadden J, Dellagostin OA. (2006) 'Immunogenicity of Mycobacterium bovis BCG expressing Anaplasma marginale MSP1a antigen'. ELSEVIER SCI LTD VACCINE, 24 (37-39), pp. 6332-6339.
    doi: 10.1016/j.vaccine.2006.05.028
  • Jeynes JCG, Mendoza E, Chow DCS, Watts PCR, McFadden J, Silva SRP. (2006) 'Generation of chemically unmodified pure single-walled carbon nanotubes by solubilizing with RNA and treatment with ribonuclease A'. WILEY-V C H VERLAG GMBH ADV MATER, 18 (12), pp. 1598-+.
    doi: 10.1002/adma.200600362
  • Rustam T, McClean S, Newcombe J, McFadden J, Eales-Reynolds LJ. (2006) 'Reduced toxicity of lipo-oligosaccharide from a phoP mutant of Neisseria meningitidis: an in vitro demonstration'. MANEY PUBLISHING J ENDOTOXIN RES, 12 (1), pp. 39-46.
    doi: 10.1179/096805106X89116

Conference papers

  • Jenson JS, Casey R, Newcombe J, Smith M, McFadden JJ, Bodman-Smith K. (2008) 'Dendritic cell responses to C-reactive protein-opsonised Neisseria meningitidis'. WILEY-BLACKWELL PUBLISHING, INC IMMUNOLOGY, Glasgow, SCOTLAND: Annual Congress of the British-Society-of-Immunology 125, pp. 124-124.
  • Casey R, McFadden J, Newcombe J, Bodman-Smith M, Bodman-Smith K. (2007) 'C-reactive protein binds to Neisseria meningitidis and affects macrophage responses to infection'. BLACKWELL PUBLISHING IMMUNOLOGY, Glasgow, SCOTLAND: Annual Congress of the British-Society-of-Immunology 120, pp. 20-20.

Mycobacterial research

The tubercle bacillus (Mycobacterium tuberculosis) infects approximately one quarter of the world's population and is responsible for three million deaths each year.

Mycobacterial research within the Microbial Sciences Group is focussed on understanding the pathogenic mechanisms that allow Mycobacterium tuberculosis to cause about three million deaths each year, and development of new vaccines to treat the disease.

We have recently initiated a new BBSRC-funded project to develop metabolic models of the TB bacillus and use these models to predict novel drug targets, particularly in persistent organisms. A web version of our model can be found here.

We currently have a Wellcome Trust-funded vacancy (to start autumn 2009) for a bioinformaticist to develop metabolic models of the TB bacillus.

Meningococcal research